Extração de dna

International Journal of Systematic and Evolutionary Microbiology (2001), 51, 687–697

Printed in Great Britain

Polyphasic taxonomy of the basidiomycetous yeast genus Rhodosporidium : Rhodosporidium kratochvilovae and related anamorphic species
! ! Jose Paulo Sampaio,1 Mario Gadanho,1 Sofia Santos,2 2,3 ! ! Filomena L. Duarte, Celia Pais,3 Alvaro Fonseca1 and Jack W. Fell4
Author for correspondence : Jose! Paulo Sampaio. Tel : j351 21 2948300. Fax : j351 21 2948530. e-mail : jss!mail.fct.unl.pt

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Centro de Recursos ! Microbiologicos, Seccao : 4 ! Autonoma de Biotecnologia, Faculdade # de Ciencias e Tecnologia, Universidade Nova de Lisboa, 2825-114 Caparica, Portugal Estacao Vitivinı! cola : 4 Nacional, INIA, 2565-191 Dois Portos, Portugal # Centro de Ciencias do Ambiente, Departamento de Biologia, Universidade do Minho, 4710-057 Braga, Portugal Rosenstiel School of Marine and Atmospheric Science, University of Miami, 4600 Rickenbacker Causeway, Key Biscayne, FL 33419, USA

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The phenotypic and genetic heterogeneity of the basidiomycetous yeast species Rhodosporidium kratochvilovae was investigated in a group of recent isolates and collection strains. A polyphasic taxonomic approach was followed which included micromorphological studies, nuclear staining, determination of sexual compatibility, physiological characterization, comparison of electrophoretic isoenzyme patterns, PCR fingerprinting, determination of mol % GMC, DNA–DNA reassociation experiments and 26S and ITS rDNA sequence analysis. The results allowed a more natural circumscription of the species, both from the genetic and phenotypic perspectives. The relationships with anamorphic species of the genus Rhodotorula were studied and isolates previously identified as Rhodotorula glutinis were found to belong to Rhodosporidium kratochvilovae. Other isolates included in the study were found to represent members of Rhodotorula glutinis var. dairenensis. Rhodosporidium kratochvilovae was found to include