Relações humanas e ética no trabalho

Brazilian Journal of Microbiology (2010) 41: 365-367
ISSN 1517-8382

DETECTION OF Brucella ovis IN OVINE FROM PARAÍBA STATE, IN THE NORTHEAST REGION OF BRAZIL
Clebert José Alves1*, Salomão Moreira de Figueiredo1, Sérgio Santos de Azevedo1, Inácio José Clementino1, Lara Borges
Keid2, Sílvio Arruda Vasconcellos2, Carolina de Sousa Américo Batista2, Vivianne Cambuí Mesquita Rocha2, Severino
Silvano Higino1
1

Unidade Acadêmica de Medicina Veterinária, Centro de Saúde e Tecnologia Rural, Universidade Federal de Campina Grande,
Patos, PB, Brasil; 2Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e
Zootecnia, Universidade de São Paulo, SP, Brasil.
Submitted: September 29, 2008; Returned to authors for corrections: October 31, 2008; Approved: November 07, 2009.

ABSTRACT
To determine the presence of Brucella ovis in ovine from Paraíba State, in the Northeast region of Brazil, 80 animals slaughtered in the public slaughterhouse of Patos city were used. Before slaughter, blood samples were collected by jugular venopuncture from each animal, and after slaughter, testicles, epidydimus and uterus were aseptically collected. For the serological diagnosis of B. ovis and B. abortus infections, the agar gel immunodiffusion (AGID) and Rose Bengal (RBT) tests were carried out, respectively. In addition, microbiological culture and polymerase chain reaction (PCR) were performed on testicle, epidydimus and uterus samples. Six animals (7.5%) tested positive for the presence of B. ovis antibodies and all animals tested negative for the presence of B. abortus antibodies. One AGID-positive animal tested positive at uterine swab culture. PCR was able to amplify DNA of Brucella spp. from the pool of testicle, epidydimus and uterus samples from AGID-positive animals. This is the first report of isolation and detection of B. ovis DNA by PCR in ovine from the Northeast region of Brazil.
Key words: Brucella ovis,