Novos horizontes para os genomas

DOE/SC-0071

New Horizons in Genomics
U.S. Department of Energy Joint Genome Institute

Santa Fe, New Mexico March 30-April 1, 2003

Prepared for the Joint Genome Institute U.S. Department of Energy Office of Science Office of Biological and Environmental Research JGI Production Genomic Facility 2800 Mitchell Drive Walnut Creek, CA 94598

Prepared by Human Genome Management Information System 1060 Commerce Park Oak Ridge National Laboratory Oak Ridge, TN 37830 Managed by UT-Battelle, LLC For the U.S. Department of Energy Under contract DE-AC05-00OR22725

Contents
New Horizons in Genomics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 Sequencing Resources . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

1

Plasmidomics: Cloning Naturally Occurring Plasmids for Sequencing and Functional Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Anne Marie Erler, Patricia Sobecky, Gary L. Andersen, and Peter Agron

2

Shotgun Library Utilization for Sequencing Projects at the JGI . . . . . . . . . . 3
Chris Detter, Eileen Dalin, Jamie M. Jett, Doug Smith, Jenna Morgan, Hope Tice, Saima Shams, Corey Chinn, Eddy Rubin, and Paul M. Richardson

3

BAC Libraries for Whole-Genome Sequencing, Comparative Genomics and Haplotype Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Pieter J. de Jong, Baoli Zhu, Mikhail Nefedov, Chung-Li Shu, Yuko Yoshinaga, and Kazutoyo Osoegawa

4

Using Transposons to Resolve Repeats in BAC Clones . . . . . . . . . . . . . . . . . 5
Jamie Jett, Jeremy Schmutz, Eileen Dalin, Jane Grimwood, Corey Chinn, Doug Smith, Susan Lucas, Chris Detter, Paul Richardson, and Eddy Rubin

5

Single Molecule DNA Sequence Profiling In Zero-Mode Waveguides Using -Phosphate Linked Nucleotide Analogs . . . . . . . . . . . . . . . . . . . . . . . 5
Jonas Korlach, Michael Levene, Mathieu Foquet, Stephen W.