Fatores de virulencia

Pesq. Vet. Bras. 33(2):241-246, fevereiro 2013

Detecção de fatores de virulência de Escherichia coli e análise de Salmonella spp. em psitacídeos1
Isadora M. de O. Corrêa2, Fernanda Flores2, Gustavo H. Schneiders2,
Larissa Q. Pereira2, Benito G. Brito3 e Maristela Lovato2*

ABSTRACT.- Corrêa I.M.O, Flores F., Schneiders G.H., Pereira L.Q., Brito B.G. & Lovato M.
2013. [Detection of virulence factors in Escherichia coli and analysis of Salmonella spp. in psittacines.] Detecção de fatores de virulência de Escherichia coli e análise de Salmonella spp. em psitacídeos. Pesquisa Veterinária Brasileira 33(2):241-246. Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Av. Roraima
1000, Santa Maria, RS 97105-900, Brazil. E-mail: [e-mail]
The enteric flora of psittacines is mainly composed of Gram positive bacteria. Gram negative bacteria, like Escherichia coli and Salmonella spp., have a high pathogenic potential and can be considerate as an indicative of management problems that may culminate in disease manifestation due to stress factors, poor diets and overcrowding, in combination with a high bacterial load on the environment. The objective of this study was evaluated the presence of Salmonella spp., Escherichia coli and the virulence genes iss and iutA from
E. coli isolates. Forty-four samples were analyzed from psittacines living in captivity, which fifteen samples were from organs fragments of necropsied birds, and twenty-nine were from cloacal and crop swabs of red-spectacled parrots (Amazona pretrei) keeping in captivity. No samples were positive for Salmonella spp. In the samples in which E. coli was detected, both virulence factors (genes iss and iutA) were present.
INDEX TERMS: Escherichia coli, Salmonella spp., genes iss, iutA, Amazona pretrei, PCR, psittacines.

RESUMO.- A flora entérica dos psitacídeos é composta principalmente por bactérias Gram positivas. Bactérias
Gram negativas,