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Detection of Extended-Spectrum β -Lactamase and Klebsiella pneumoniae Carbapenemase Genes Directly from Blood Cultures by Use of a Nucleic Acid Microarray
Joel T. Fishbain, Oleg Sinyavskiy, Kathleen Riederer, Andrea M. Hujer and Robert A. Bonomo J. Clin. Microbiol. 2012, 50(9):2901. DOI: 10.1128/JCM.01023-12. Published Ahead of Print 20 June 2012.

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Detection of Extended-Spectrum -Lactamase and Klebsiella pneumoniae Carbapenemase Genes Directly from Blood Cultures by Use of a Nucleic Acid Microarray
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Joel T. Fishbain,a Oleg Sinyavskiy,a Kathleen Riederer,a Andrea M. Hujer,b,c and Robert A. Bonomob,c,d,e
Departments of Medicine and Graduate Medical Education, St. John Hospital and Medical Center, Detroit, Michigan, USAa; Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio, USAb; Research Service, Louis Stokes Cleveland Veterans Affairs Medical Center, Cleveland, Ohio, USAc; and Departments of Molecular Biology and Microbiologyd and Pharmacology,e Case Western Reserve University School of Medicine, Cleveland, Ohio USA

The growing crisis of multidrug-resistant (MDR) Gram-negative bacteria requires that current technologies permit the rapid detection of extended-spectrum -lactamase (blaESBL) and Klebsiella pneumoniae carbapenemase (blaKPC) genes. In the present study, we assessed the performance characteristics of a commercially available nucleic acid microarray system for the detection of blaESBL and blaKPC genes directly from positive blood cultures. Using blood cultures (BCs) that contained Gram-negative bacilli identified by Gram staining, we isolated bacterial DNA using spin columns (BC-C) and rapid water lysis (BC-W). Twenty