Optimização de processos fermentativos em actinomicetos

Hindawi Publishing Corporation Journal of Biomedicine and Biotechnology Volume 2010, Article ID 141876, 10 pages doi:10.1155/2010/141876

Research Article Optimization of the Fermentation Process of Actinomycete Strain Hhs.015T

Xinxuan Wang, Lili Huang, Zhensheng Kang, Heinrich Buchenauer, and Xiaoning Gao
College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling 712100, China Correspondence should be addressed to Lili Huang, [e-mail] Received 25 October 2009; Revised 28 February 2010; Accepted 11 March 2010 Academic Editor: Elvira Gonzalez De Mejia Copyright © 2010 Xinxuan Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Strain Hhs.015T (Saccharothrix yanglingensis sp. nov.), an antagonistic endophytic Saccharothrix actinomycete isolated from roots of cucumber plants, exhibited a broad antimicrobial spectrum in vitro and was active as a biocontrol against plant diseases in field trials. The SSY medium was used for production of antimicrobial metabolites by strain Hhs.015T . However, this medium is too expensive for large-scale production. In this study, an alternative culture medium, based on agricultural waste products (e.g., apple pomace), was optimized. The results showed that the alternative medium contained 15 g apple pomace, 4 g rapeseed meal, 0.1 g KH2 PO4 , and 0.6 g MgSO4 · 7H2 O in 1 L distilled water. This medium reduced the material costs by 91.5% compared to SSY medium. Response surface methodology (RSM) was used to investigate the influence of environmental variables on production of compounds of antimicrobial metabolites. The optimal conditions achieved were initial pH 7.0, medium volume of 90 mL in 250 mL flasks, rotary speed of 100 rpm, temperature 25◦ C, and inoculation volume of