Hippocampal proteomic profile in temporal lobe epilepsy

Journal of

Epilepsy and
Clinical
Neurophysiology
J Epilepsy Clin Neurophysiol 2012;18(2):53-56

Hippocampal Proteomic Profile in Temporal Lobe Epilepsy*
Persike DSa, Lima MLb, Amorim RPa, Cavalheiro EAa, Yacubian EMTa, Centeno RSa, Carrete Jr Ha,
Schenkman Sb, Canzian Mc, Fernandes MJSa
Universidade Federal de São Paulo (UNIFESP)

ABSTRACT
In this study we used proteomics approaches to obtain the protein profile of human epileptic hippocampi (N=6) and control hippocampi obtained from autopsy (N=6). We used two-dimensional gel electrophoresis (2-D) coupled to HPLC and Mass spectroscopy (MALDI-TOF) to identify proteins differentially expressed. Nine proteins were differentially expressed comparing the hippocampus of patients with the hippocampus of control.
Proteins that were increased in the hippocampus of patients with TLE were: isoform 1 of serum albumin, HSP 70, dihydropyrimidinase-related protein 2, isoform 1 of myelin basic protein, proton ATPase catalytic subunit A, and dihydrolipoyllysine-residue acethyltransferase component of pyruvate dehydrogenase complex. The expression of isoform 3 of spectrin alpha chain (fodrin) was down-regulated while the proteins glutathione S-transferase
P and the DJ-1 (PARK7) were detected only in the hippocampus of patients with TLE. Taken together, our results provide evidence supporting a direct link between metabolic disturb and oxidative damage related to pathophysiology of TLE. Besides, indicates biomarkers for further investigations as therapies targeted to epilepsy. Keywords: temporal lobe epilepsy, hippocampus, proteomics, biomarkers.
RESUMO
Perfil proteômico hipocampal em epilepsia do lobo temporal
No presente estudo empregou-se o método de proteômica para obter a expressão diferencial de proteínas em hipocampo de pacientes com epilepsia do lobo temporal (ELT) (N=6) comparado a hipocampos controle obtidos por meio de autópsia (N=6). O estudo foi feito por meio de eletroforese bidimensional,