Synergistic antibacterial activity of the essential oil of aguaribay (schinus molle l.)

antibacterial essential oil extracted from the leaves and fruits. This work reports a complete study of its chemical composition and determines the antibacterial activity of Schinus molle L. essential oil and its main components. The results showed that the crude extract essential oil has a potent antibacterial effect on Staphylococcus aureus ATCC 25923, a strong/moderate effect on Escherichia coli ATCC 25922 and moderate/weak one on Pseudomonas aeruginosa ATCC 27853. Keywords: essential oil; antibacterial activity; Schinus molle L.; agar diffusion; effect of vapor method of cylindrical cavities; MIC; terpinenediols; 7-formyloxysabinen-2-ol 1. Introduction Essential oils are natural and complex volatile compounds [1,2], characterized by a strong odor, generally soluble in organic solvents with lower density than water [3,4]. They are produced in aromatic and medicinal plants as secondary metabolites and are obtained from various plant parts Molecules 2012, 17 12024 (flower, seed, leaves, twigs, bark, herbs, wood, fruits and roots) [3] and stored in secretory cells, cavities, vessels, or epidermal cells called glandular trichomes [4]. The family Anacardiaceae of pantropical occurrence includes a few representatives in temperate regions. The family comprises about 70 genera and 600 species. They are used traditionally as a healing, stomachic and antidiarrheal agent, due to the presence of tannins and oil resins [5]. In this family, Schinus…

em água de irrigação de hortaliças. Palavras-chave: Antibacteriano; alimento; bioproduto Propolis is a mixture of resinous and balsamic substances and balsamic that bees of the species Apis mellifera L. collect from various plants and lead to the hive. This resin has been widely used for medical applications, resulting in an increased interest for its chemical composition, as well as its botanical origin It can be found in many regions of the world, with wide variation in chemical…

add 45 mL of glacial acetic acid. Plug flask with cotton wool, boil again, cool and filter. (stain and fixative, good for protozoa and nuclei) Aceto-Orcein Staining Solution Heat 31.5 mL of glacial acetic acid and 13.5 mL of DI water almost to boiling. When acid is hot, add 2 g of synthetic orcein and allow to cool. Dilute by adding 55 mL of DI water; stir and filter. (connective tissue stain) Adrenaline Hydrochloride Dissolve 0.1 g of adrenaline hydrochloride in 100 mL of Ringer’s…

study was carried out to provide subsidies for sanitary actions applied to manipulators, environments and surfaces, assessing levels of microbiological contamination in three commercial restaurants (A, B and C) in Viçosa, Minas Gerais State. Microbiological analysis were performed for presumptive counting of Bacillus cereus and mesophilic aerobic bacteria on surfaces of stainless steel benches, equipments, utensils and hands of the manipulators (swab technique), and air (simple…

Para leitura dos resultados é utilizada a tabela do número mais provável de Speak [5]. Dos tubos que apresentarem turvação e gás no tubo de Durhan, alíquotas devem ser semeadas com auxílio de alça bacteriológica, pela técnica de esgotamento por estrias descontínuas, em placas de petri com meio de Teague- (EMS) Agar Eosina Azul Metileno e ser incubadas a 35-37ºC por 24horas. Para identificação de E.coli, as colônias devem ser submetidas às provas bioquímicas nos…

process and their respective certification. The methods of choice for the validation should be sensitive to detect traces of contaminants, should preferably be easy to execute, deliver results fast, safe and effective and ensure a high degree of reliability of the results of the validation process. The aim of this study was the microbiological monitoring of equipment and utensils used for handling of drugs in FarmaUSCS to subsequently develop appropriate methodology to implement a…

(Johnson & Curl, 1972). Um mL da solução do solo (1:100), com cinco repetições por tratamento, foi plaqueado utilizando o meio semi-seletivo de Nash & Snyder (1962). A estimativa do número de propágulos Fusarium spp. por grama de solo foi obtida pela fórmula, NP= NCx100, onde NP= nº de propágulos por grama de solo e NC= número médio de colônias/placa. Não foi realizada a estimativa de população de cada espécie de Fusarium. Para a análise de variância os dados foram transformados para…

physicochemical (pH, acidity, chemical composition and coagulation time) and psychrotrophic bacteria raw milk profiles of holstein and Girolando cow breeds. From each breed, 30 samples of raw milk were analyzed to evaluate the physicochemical profile and 16 samples to determine the psychrotrophic bacteria profile. Sam ples were collected after complete milking of cows between the 3rd and 4th months of lactation. The milk of Girolando animals had higher fat content and shorter…

This study assessed the physicochemical and microbiological characteristics of raw milk came from tanks from Curvelo region, Minas Gerais, Brazil, and evaluating the growth of psychrotrophic in raw milk refrigerated at temperatures of 4.7 and 10° C. It was found that 9.09% of the tanks were not in compliance with the legislation regarding the cryoscopic index and 22.72% for the Dornic acidity. The physical-chemical tests found no presence of hydrogen peroxide, formaldehyde, or alkaline…

.... xii 16 20 33 49 57 59 92 106 108 LISTA DE ABREVIATURAS E SÍMBOLOS % ºC pH FES FL Fig. Tab. h MA mL > < cfu RMN-13C RMN de 13C DEPT CG-EM EMBRAPA BDA PVC min v/v rpm ppm Cr0 Cr12 Cr24 CCD UV CDCl3 TMS AGME IE-EM H2SO4 MeOH d.i. Rt K Porcentagem Graus Celsius Potencial Hidrogeniônico Fermentação em Estado Sólido Fermentação Líquida Figura Tabela Horas Ministério da Agricultura Mililitro Maior que Menor que …