A importância da didática como ferramenta na construção do conhecimento

Vol. -1 , Issue -3, Year - June 2012 ISSN 0976-9692

METHOD DEVELOPMENT AND VALIDATION FOR THE GC ASSAY OF á-PINENE IN TEA TREE OIL FORMULATION
Nikhil Gulati1, Amit K. Goyal1, K.S. Rajesh1, Karan mittal1, G. Rath1
1

Department of Pharmaceutics, ISF College of Pharmacy, Moga (Punjab), India

Abstract The present study was oriented towards the development and validation of Gas chromatography-flame ionization detection (GC-FID) method for pure Tea tree oil (TTO). Separation was achieved by Stationary phase ECTM- WAX, coated with polyimide that gives notorious flexibility of the capillary column, Film thickness (0.25µm), length 30m x0.25mm i.d and the Injector and detector temperature, 230 and 240oC. The data was acquired by data processing system, Nuchrom GC Software. The samples and standard were prepared with dichloromethane. The calibration curve for á- pinenewas linear (r2= 0.998) from 0.1% to 0.5% range of the analytical concentration of pureá- pinene oil. The optimal separation was achieved at 9.69 minute.% R.S.D for the intra-day and inter-day precisions was found to be 0.949 and 0.781 respectively. The limits of detection and quantitation were determined to be 0.0003 % and 0.001 % respectively. Analytical performance demonstrated that the proposed method is highly sensitive, precise and accurate and hence was successfully applied for the quantification ofá- pinenein formulation of TTO. Keywords: Tea Tree oil, GC, Validation.

Introduction Tea tree oil (Melaleuca alternifolia) is distilled from the fronds of a tree native to New South Wales, Australia, and parts of New Zealand. This tree, M. alternifolia, is a member of the myrtaceae family, and is extremely hardy and disease resistant. The leaves have been employed medicinally for centuries by New South Wales Aborigines, and the name is said to stem from a visit by Captain Cook, whose crew made a tea from the leaves. Tea tree oil has been proven to be a powerful yet natural antiviral, antibacterial,