Reproduçao piometra em caes

Kafkas Univ Vet Fak Derg
16 (2): 313-318, 2010

RESEARCH ARTICLE

Investigation on the Antiendotoxic Effect of the Combination of

Polymyxin E and Ampicillin in Dogs with Endotoxic Pyometra

M. Ayşe DEMİREL *

Şükrü KÜPLÜLÜ *

* Department of Obstetric and Gynecology, Faculty of Veterinary Medicine, University of Ankara, 06110 Diskapi,
Ankara - TURKEY

Makale Kodu (Article Code): KVFD-2009-730
Summary
The aim of this study was to determine the incidence and levels of the gram negative bacterial endotoxin, and to investigate the effects of antiendotoxemic treatment along with operation. Thirty bitches with pyometra were used in this study. The bitches were randomly allocated into two groups as Group I (treated with polymyxin E-ampisillin) and Group II (treated with ampisillin). Samples for bacteriological examination were taken from vagina, vesica urinaria and uterus of all bitches. Blood samples were collected to evaluate biochemical, haematological tests and serum toxine levels before the operation and on 7 days after operation. Gelation was carried out to determine the gram negative bacterial endotoxin level with E-toxate test kit in accordance with gelation system. Gram negative bacteria were isolated 66% in Group I and 80% Group II. Escherichia coli were dominantly (63.4%) isolated from the uteri of 30 bitches with pyometra. Before the operation, serum toxin levels were found in Group I (n=10) 1.2±0.4 EU/ml and in Group II (n=12) 0.9±0.2 EU/ml.
Five bitches in Group I and 3 bitches in Group II died on the third day after operation. Survival rate was a positive correlation with toxin levels (r=0.727;