Mitocondria em adipocitos

J Physiol Biochem DOI 10.1007/s13105-011-0074-6

ORIGINAL PAPER

Dynamic regulation of mitochondrial network and oxidative functions during 3T3-L1 fat cell differentiation
Pierre-Henri Ducluzeau & Mélanie Priou & Mireille Weitheimer & Melissa Flamment & Lucie Duluc & Fransceco Iacobazi & Raffaela Soleti & Gilles Simard & Annie Durand & Jennifer Rieusset & Ramaroson Andriantsitohaina & Yves Malthièry

Received: 22 June 2010 / Accepted: 7 January 2011 # University of Navarra 2011

Abstract Mitochondria have been shown to be impaired in insulin resistance-related diseases but have not been extensively studied during the first steps of adipose cell development. This study was designed to determine the sequence of changes of the mitochondrial network and function during the first days of adipogenesis. 3T3L1 preadipocytes were differentiated into adipocytes without using glitazone compounds. At days 0, 3, 6, 9, and 12, mitochondrial network imaging, mitochondrial oxygen consumption, membrane potential, and oxidative phosphorylation efficiency were assessed in permeabilized cells. Gene and protein expressions related to fatty acid metabolism and mitochondrial network were also determined. Compared to preadipocytes (day 0), new adipocytes (days 6 and 9) displayed profound changes of their mitochondrial network that underwent fragmentation and redistribution around lipid droplets. Drp1 and mitofusin 2 displayed a progressive increase in their gene expression and protein content during the first 9 days of differentiation. In parallel with
P.-H. Ducluzeau (*) : M. Priou : M. Weitheimer : M. Flamment : L. Duluc : F. Iacobazi : R. Soleti : G. Simard : R. Andriantsitohaina : Y. Malthièry INSERM U694, Univerité d’Angers, CHU Angers, 4 rue Larrey, Angers 49033, France e-mail: [e-mail] A. Durand : J. Rieusset INSERM 870, INRA 1235, Université Lyon 1, Hospices Civils de Lyon, Lyon, France

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